The Effect of Injection with Papaya (Carica Papaya L.) Seed Extract on Sperm Concentration and Spermatogenic Cells of Male Rats (Rattus norvegicus L.) Strain LMR.
So far men as a subject in family planning program had no priority, however recently men become a focus. Established mothodes for male contraception are through condom and vasectomy. Using condoms create psychological complaints, whereas vasectomy although very effective has often permanent effect. An other method of contraception is hormonal; besides that it is important to develop contraception using plants with antifertility effect such as papaya seed. Therefore, the aim of this research is to know the effect of extract papaya seeds on concentration and viability of sperms in vas deferens of male rat Strain LMR. This research was done using papaya seed extract, Bangka variety with 7 treatments, doses/kg/body weight, including 0 mg; 0.1 mg; 0.5 mg; 0.9 mg; 1.0 mg; 5.0 mg; 9.0 mg for times each treatment. Administration of papaya seed extract was performed by intramusculary injection for 20 days (1,5 seminiferous epithelium cycles). Investigation were done on 1) sperms concentration of vas deferens, 2) weight of testis, 3) seminiferous tubules diametric, 4) condition of spermatogenic cells. Injection with papaya seed extract for 20 days increased sperm concentration of vas deferens significantly (P<0,01), decreased population of spermatogonium A and primary spermatocytes preleptoten significantly (p<0,05), did not give any significant effect on weight of testis, seminiferous tubules diametric, primary spermatocytes pachyten and spermatid (P>0,05)
sperms concentration; weight of testis; seminiferous tubules diametric; spermatogenic cells; contraception